Biolegend, 850102, Purified anti-Dopamine Receptor D4 Antibody, 100μg

Dopamine Receptor D4 (DRD4) is a G-protein coupled receptor which inhibits adenyl cyclase activity. It is linked to many neurological and psychiatric conditions including schizophrenia and bipolar disorder, addictive behaviors, Parkinson’s disease, and eating disorders such as anorexia nervosa. DRD4 mutations have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: A peptide corresponding to the 3rd extracellular domain of DRD4 (a.a. 176-185).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: IHC result in mouse is much weaker than in human at 5 µg/mL. This antibody does not cross react with DRD1 in Western Blot.
Application References(PubMed link indicates BioLegend citation): Wolstencroft EC, et al. 2007. J. Mol. Histol. 38(4):333. (WB, IF)
Product Citations: Martin CA, et al. 2022. Regen Biomater. 9:rbac031. PubMed
RRID: AB_2715868 (BioLegend Cat. No. 850101) AB_2715869 (BioLegend Cat. No. 850102)
Structure: Dopamine Receptor D4 is a 467 amino acids protein with a molecular mass of ~48 kD.
Distribution: Tissue distribution: Mainly in the Central Nervous System. Highly expressed in Retina. Cellular distribution: Mainly at plasma membrane.
Function: DRD4 is activated by the neurotransmitter dopamine. It is responsible for neuronal signaling in the mesolimbic system. DRD4 inhibits adenylyl cyclase, and has been reported to modulate the circadian rhythm of contrast sensitivity via modulating the rhythmic expression of NPAS2 in the retinal ganglion cells.
Interaction: DRD4 forms homo- and hetero-oligomers with Dopamine Receptor D2, which may modulate agonist-induced downstream signaling. DRD4 may also interact with ADORA2A and KLHL12.
Ligand/Receptor: Dopamine.
Cell Type: Dopaminergic Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family: GPCR, Postsynaptic proteins
Antigen References: 1. Pappa I, et al. 2015. Neurosci. Biobehav. Rev. 57:175. 2. Yildirim BO, Derksen JJ. 2015. Psychiatry Res. 229(3):633-77.
Gene ID: 1815
UniProt: View information about Dopamine Receptor D4 on UniProt.org
Clone: 2B9/DRD4
Regulatory Status: RUO
Other Names: DRD4, Dopamine D4 receptor
Isotype: Mouse IgG1, κ