Biolegend, 851205, Alexa Fluor® 647 anti-Tau, 368-441 Antibody, 25μg

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant C-terminal fragment of human 4R Tau protein encompassing amino acid residues 231-441.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This antibody cross-reacts with human 3R and 4R Tau isoforms, and recognizes endogenous human Tau in brain lysates by WB. The antibody has minimal reactivity with mouse Tau protein.
RRID: AB_2801195 (BioLegend Cat. No. 851205) AB_2801196 (BioLegend Cat. No. 851206)
Structure: Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution: Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle. Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.
Function: Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction: Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Tau
Antigen References: Augustinack JC, et al. 2002. Acta Neuropathol. 103(1):26-35. PubMed. Seubert P, et al. 1995. J Biol Chem. 270(32):18917-22.PubMed. Dong Y, et al. 2012. PLoS One 7(6):e39386. PubMed.
Gene ID: 4137
UniProt: View information about Tau 368-441 on UniProt.org
Clone: A16097F
Regulatory Status: RUO
Other Names: Microtubule associated protein tau
Isotype: Rat IgG2a, λ