Biolegend, 852202, Purified anti-TPP1 Antibody, 100μg

TPP1 is a member of the sedolisin family of serine proteases. The proenzyme is activated in the lysosome by auto-proteolysis where it cleaves tripeptides from the N-termini of substrates. Mutations of TPP1 are associated with late-infantile neuronal ceroid lipofuscinosis and Spinocerebellar Ataxia, Autosomal Recessive 7.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant mature human TPP1 expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not recognize rodent (mouse or rat) TPP1.
Application References(PubMed link indicates BioLegend citation): Wujek P, et al. 2004. J. Biol. Chem. 279:12827-39.
RRID: AB_2728589 (BioLegend Cat. No. 852201) AB_2728590 (BioLegend Cat. No. 852202)
Structure: Human TPP1 is a 563 amino acid protein with a molecular mass of 61 kD. An isoform of 34.5 kD and 320 amino acids (244-563) is generated by alternative splicing.
Distribution: Tissue sources: Ubiquitously expressed. Highest levels in heart, placenta, bone marrow, lung, pineal, and lymphocytes. Cellular distribution: Lysosome and extracellular.
Function: TPP1 cleaves tripeptides from the N-termini of substrates in lysosomes and has endopeptidase activity.
Interaction: CLN5.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: Getty AL, Pearce DA. 2011. Cell Mol. Life Sci. 68:453-74. Cárcel-Trullols J, et al. 2015. Biochim. Biophys. Acta. 2242-55. Kyttälä A, et al. 2006. Biochim Biophys Acta. 920-33.
Gene ID: 1200
UniProt: View information about TPP1 on UniProt.org
Clone: 2E12
Regulatory Status: RUO
Other Names: Tripeptidyl Peptidase 1, Tripeptidyl Aminopeptidase, TPP-1, LPIC, Ceroid-Lipofuscinosis, Neuronal 2, Spinocerebellar Ataxia, Autosomal Recessive 7, Tripeptidyl-Peptidase 1, Tripeptidyl-Peptidase I, TPP I , SCAR7, GIG1
Isotype: Mouse IgG2b, κ