Biolegend, 853301, Purified anti-TMEM119 (Extracellular) Antibody, 25μg

Microglia are the resident parenchymal myeloid cells of the CNS, and play an important role in development, homeostasis, disease, and injury. TMEM119 is a transmembrane protein and was identified as a microglia-specific marker in both mouse and human CNS. Moreover, TMEM119 immunoreactivity has been exclusively detected on a subset of Iba1(+) CD68(+) microglia with ramified and amoeboid morphologies in the brains of neurodegenerative diseases. TMEM119 also plays an important role in bone formation and normal bone mineralization. It promotes the differentiation of myoblasts into osteoblasts. and it may induce the commitment and differentiation of myoblasts into osteoblasts. TMEM119 is also essential for normal spermatogenesis and late testicular differentiation.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human TMEM119 protein (extracellular domain)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested WB, ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.6 - 5.0 µg/mL. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For microscopy, the antibody was found to work best when using fresh frozen human tissues. Communication by Dr. Frederick Christian Bennett at Stanford University, under review in Neuron. *The human TMEM119 full length plasmid was a courtesy gift from Dr. Ben Barres at Stanford University.
Application References(PubMed link indicates BioLegend citation): Bennett FC, et al. 2018. Neuron. 98:1-14 PubMed
Product Citations: Tang Y, et al. 2023. J Clin Invest. 133:. PubMed Patel T, et al. 2022. Aging Cell. 21:e13606. PubMed Bodnar B, et al. 2021. Front Cell Neurosci. 15:682272. PubMed Kumar A, et al. 2021. EBioMedicine. 63:103192. PubMed Rai MA, et al. 2020. Retrovirology. 17:35. PubMed Ruan C, et al. 2020. Brain Behav Immun. 83:180. PubMed Bennett FC, et al. 2018. Neuron. 98:1170. PubMed Chen P, et al. 2020. Cancer Discov. 0.674305556. PubMed Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed Toedebusch RG, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID: AB_2734646 (BioLegend Cat. No. 853301) AB_2734647 (BioLegend Cat. No. 853302)
Structure: TMEM119 is an 283 amino acid protein with a molecular mass of 30 kD.
Distribution: Tissue distribution: TMEM119 is expressed in the brain, bone, and lymphoid tissues. Cellular distribution: Membrane and endoplasmic reticulum.
Function: TMEM119 is an important microglia marker and is implicated in neurodegenerative diseases.
Interaction: TMEM119 interacts with SMAD1, SMAD5 and RUNX2.
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References: Bennett ML, et al. 2016. Proc Natl Acad Sci U S A. 113 Satoh J, et al. 2016. Neuropathology. 36:39-49 Kawao N and Kaji H. 2015. J Cell Biochem. 116:687 Zrzavy T, et al. 2017. Brain. 140:1900-1913
Gene ID: 338773
UniProt: View information about TMEM119 on UniProt.org
Clone: A16075D
Regulatory Status: RUO
Other Names: Transmembrane protein 119
Isotype: Mouse IgG2b, κ