Biolegend, 853602, Purified anti-TOMM40 Antibody, 100μg

Mitochondria fulfill central functions in cellular energetics, metabolism, and signaling. The outer membrane translocator complex (the TOM complex) imports most mitochondrial proteins, and TOMM40 is the key subunit of the complex, the pore through which the vast majority of imported proteins must pass. TOMM40 possesses chaperone-like properties, which helps expedite movement of the pre-proteins through the channel. TOM cooperates with the TIM23 (Translocase of the Inner Membrane 23) complexes to import matrix-targeted proteins. TOMM40 is implicated in many neurodegenerative diseases; reduced TOMM40 mRNA expression in Alzheimer’s disease patients was shown to correlate with cognitive decline.
100μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: A synthetic peptide corresponding to TOMM40 protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody shows negligible cross-reactivity with human TOMM40.
RRID: AB_2749940 (BioLegend Cat. No. 853601) AB_2749941 (BioLegend Cat. No. 853602)
Structure: TOMM40 is a 361 amino acid protein with a molecular weight mass of ~40 kD.
Distribution: Tissue distribution: TOMM40 is expressed in numerous tissues including the central nervous system (CNS), muscle, and the immune systems. Cellular distribution: Cytosolic, mitochondria, and nucleus.
Function: TOMM40 is the key subunit of the TOM complex, which imports proteins into the mitochondria.
Interaction: TOMM40 Interacts with mitochondrial targeting sequences and the TIM complex.
Biology Area: Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Transcription Factors
Molecular Family: Mitochondrial Markers
Antigen References: Gottschalk WK, et al. 2014. J. Parkinsons Dis. Alzheimers Dis. 12. Shiota T, et al. 2015. Science 349:1544-8. Mise A, et al. 2017. J. Alzheimers Dis. 60:1107-1117. Ahting U, et al. 2001. J. Cell. Biol. 153:1151-60.
Gene ID: 10452
UniProt: View information about TOMM40 on UniProt.org
Clone: A17123A
Regulatory Status: RUO
Other Names: Mitochondrial import receptor subunit TOM40 homolog
Isotype: Mouse IgG2b, κ