Biolegend, 853701, Purified anti-Synapsin I/II/III Antibody, 25μg

Synapsins are a family of proteins involved in neurotransmitter release at the chemical synapses. Synapsins anchor synaptic vesicles to the cytoskeletal structures at the presynaptic terminal. Upon receiving an action potential, phosphorylation of synapsins, possibly by PKA, leads to the release of synaptic vesicles from cytoskeletal structures. This allows membrane docking and fusion of synaptic vesicles with the presynaptic membrane and neurotransmitter release.
25μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein corresponding to amino acids 362-450 of human Synapsin I
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.005 µg per ml. For immunohistochemistry, a concentration range of 0.1 - 0.5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Anastas JN, et al. 2020. Cancer Cell. 36(5):528-544.e10.. PubMed
RRID: AB_2749954 (BioLegend Cat. No. 853701)
Structure: Synapsin I is a 704 amino acid protein with an apparent molecular mass of ~74 kD. Synapsin II is a 582 amino acid protein with an apparent molecular mass of ~63 kD. Synapsin III is a 580 amino acid protein with an apparent molecular mass of ~63 kD.
Distribution: Tissue distribution: Mainly in the central nervous system. Also expressed in testis. Cellular distribution: Cytosol, nucleus, and plasma membrane.
Function: Synapsins are involved in the regulation of synaptic vesicle fusion and neurotransmitter release at the presynaptic terminals.
Interaction: CAPON (Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein), PKA
Cell Type: Neurons
Biology Area: Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family: Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References: Greengard P, et al. 1993. Science. 259(5096):780 Milovanovic D, et al. 2017. Neuron. 93(5):995 Evergren E, et al. 2007. J. Neurosci. Res. 85(12):2648
Gene ID: 685368548224
UniProt: View information about Synapsin I/II/III on UniProt.org
Clone: A17080A
Regulatory Status: RUO
Other Names: SYN, Synapsin, Synapsin I, Synapsin II, Synapsin III
Isotype: Mouse IgG2b, κ