Biolegend, 855902, Purified anti-RAGE Antibody, 100μg

RAGE (receptor for advanced glycation end products), is a member of the immunoglobulin super family of proteins with a molecular weight estimated at ~35 kD. RAGE is a pattern recognition receptor as it recognizes a common structure of a class of ligands, including HMGB1, S10012, S100B, S100A7, and amyloid precursor protein (APP).  RAGE may play a role in the pathogenesis of neurodegenerative disorders such as Alzheimer’s disease (AD). Increased RAGE expression levels have been observed in neurons and microglia surrounding senile plaques. In addition, RAGE can mediate the transport of amyloid beta across the blood-brain barrier and its accumulation in the brain.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human RAGE
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2783446 (BioLegend Cat. No. 855901) AB_2783447 (BioLegend Cat. No. 855902)
Structure: RAGE is a 404 amino acid protein with an apparent molecular mass of ~43 kD.
Distribution: Tissue Distribution: Lung (major) and colon (less expressed) Cellular Distribution: Plasma membrane, extracellular, and nucleus
Function: Functions as a pattern recognition receptor in the innate immune system that initiates proinflammatory responses.
Interaction: RAGE forms homo-dimers, and interacts with HMGB1, S100A1, APP, S100B, S100A12, and S100A14.
Ligand/Receptor: AGEs (advanced glycation end-products)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: Barbezier N, et al. 2014. Ann. Biol. Clin. (Paris) 72:669. Kalea AZ, et al. 2009. Clin. Sci. (Lond) 116:621.
Gene ID: 177
UniProt: View information about RAGE on UniProt.org
Clone: A17158D
Regulatory Status: RUO
Other Names: RAGE, AGER, Advanced glycosylation end product-specific receptor
Isotype: Mouse IgG2b, κ