Biolegend, 856402, Purified anti-VPS35 Antibody, 100μg

VPS35 is the largest subunit of the retromer complex, which plays an important role in retrograde protein transport from the endosome to the trans-Golgi network. VPS35 binds to VPS26 (VPS26A or VPS26B) and VPS29 to form the vacuolar protein sorting subcomplex (VPS), which in turn interacts with different nexins to form different retromer variants that transport different targets. D620N mutation in VPS35 has been implicated as a causative genetic factor for developing late-onset, autosomal dominant familial Parkinson’s disease, and could serve as potential therapeutic target for PD.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2783456 (BioLegend Cat. No. 856401) AB_2783457 (BioLegend Cat. No. 856402)
Structure: VPS35 is a 796 amino acid protein with an apparent molecular mass of ~92 kD.
Distribution: Tissue Distribution: Ubiquitously expressed in all tissues. Cellular Distribution: Cytosol, lysosome, endosome, and secreted.
Function: Largest subunit of the retromer complex involved in retrograde transport of proteins from endosomes to the trans-Golgi network.
Interaction: VPS26 (VPS26A or VPS26B), VPS29, SNX1, SNX2, IGF2R, SNX3, GOLPH3, LRRK2, SLC11A2, SNX27, RAB7A.
Biology Area: Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Organelle Markers
Antigen References: Cui Y, et al. 2018. FEBS Lett. 592(7):1096 Williams ET, et al. 2017. J Parkinsons dis. 7(2):219 Ferreira M, et al. 2017. Acta Neurol Scand. 135(3):273
Gene ID: 557376511425479
UniProt: View information about VPS35 on UniProt.org
Clone: A17084A
Regulatory Status: RUO
Other Names: Vacuolar protein sorting-associated protein 35
Isotype: Mouse IgG2b, κ