Biolegend, 856602, Purified anti-Galectin-2 Antibody, 100μg

Human Galectin-2 (Gal-2) is a soluble lactose-binding lectin that was initially cloned from a human hepatoma cDNA library, and designated as L-14-II (LGALS2). The galectins are a family of lectins that possess similar carbohydrate binding specificities and conserved consensus sequences. The mammalian galectins are involved in the regulation of inflammation, cell adhesion, cell proliferation, and cell death. Galectins are divided into three groups: prototype, tandem repeat a, and chimera type. Gal-2 belongs to the prototype group that includes Galectins 1, 2, 5, 7, 10, 11, 13, 14, and 15. The prototype galectins contain one carbohydrate recognition domain (CRD) and can form homodimers. Gal-2 shares 43% amino acid sequence identity with Gal-1. Studies in rat tissues and human tumor cell lines have shown that Gal-2 is expressed in gastrointestinal tract. Gal-2 induces apoptosis of lamina propria T lymphocytes, and ameliorates acute and chronic experimental colitis in mice. In addition, Gal-2 and Gal-1 induce phosphatidylserine exposure in fMLP-activated human neutrophils, but not in resting neutrophils. Galectin-1, -2, and -3 exhibit differential recognition of sialylated glycans, and  Gal-2 and Gal-3 possesses higher binding than Gal-1 to fucose-containing A and B blood group antigens
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Galectin-2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.1 - 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2783460 (BioLegend Cat. No. 856601) AB_2783461 (BioLegend Cat. No. 856602)
Structure: Human Galectin-2 is a 132 amino acid protein with a molecular mass of ~15 kD.
Distribution: Tissue distribution: Highly expressed in gastrointestinal tract, and to lesser extent in the immune system, kidney, and brain. Cellular distribution: Cytosol.
Function: Galectin-2 his involved in immune cell homeostasis, inflammation, cell adhesion and protein trafficking. The protein levels of Galectin-2, and also other Galectin proteins, are significantly increased in colon and breast cancer patients, promoting cancer cell adhesion.
Interaction: Interactions: GlycansCellular targets: Interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α)
Ligand/Receptor: CD14, Toll-like receptor (TLR)-4 pathway, glycoconjugates
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules
Antigen References: Delacour D, et al. 2009. Traffic. 10(10):1405-13. Barrow H, et al. 2011. Clin Cancer Res. 17(22):7035-46. Rabinovich G.A., et al. 2002. Trends Immunol. 23(6):313-20. Yıldırım C. et al. 2015. PLoS One. 10(4):e0124347.
Gene ID: 3957
UniProt: View information about Galectin-2 on UniProt.org
Clone: A17160B
Regulatory Status: RUO
Other Names: LGALS2, HL14, Lectin, CGL-2, Galactoside-Binding, Soluble, 2 Beta-Galactoside-Binding Lectin L-14-II, Lactose-Binding Lectin 2, S-Lac Lectin 2, Gal-2
Isotype: Mouse IgG2b, κ