Biolegend, 858701, Purified anti-LRP1 (CD91) Antibody, 25μg

LRP1 gene encodes a 4544 amino acid precursor protein that is processed in the trans-Golgi complex into the 515 kD alpha chain and the 85 kD beta chain. The alpha and beta chains of LRP1 form a transmembrane receptor on the plasma membrane that plays a role in receptor-mediated endocytosis and lipoprotein metabolism. LRP1 contributes to the maintenance of brain homeostasis through various signaling pathways. It also mediates the uptake and clearance of amyloid beta (Aβ). Dysfunction of LRP1 may be a contributing factor in the developent of  Alzheimer’s disease.
25μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody recognizes the beta chain of LRP1.
Application References(PubMed link indicates BioLegend citation): Spoelgen R, et al. 2009. Neuroscience. 158(4):1460 (WB) Lleó A, et al. 2005. J Biol Chem. 280(29):27303 (WB, ICC) Campana WM, et al. 2006. J Neurosci. 26(43):11197 (WB, IHC) Nieves EC and Manchanda N. 2010. J Biol Chem. 285(17):12595 (IP)
RRID: AB_2801223 (BioLegend Cat. No. 858701) AB_2801224 (BioLegend Cat. No. 858702)
Structure: The beta chain of LRP1 is a 601 amino acid protein with a molecular mass of 85 kD.
Distribution: Tissue Distribution: brain, lung, adipose tissue, and reproductive system. Cellular Distribution: plasma membrane, nucleus, cytosol, lysosome, and endosome.
Function: Receptor-mediated endocytosis; lipoprotein metabolism
Interaction: SHC1, GULP1, DAB1, LRPAP1, NPXY
Ligand/Receptor: Receptor for APOE4/APOE, Alpha-2-macroglobulin, Pseudomonas aeruginosa exotoxin A
Cell Type: Microglia, Oligodendrocytes
Biology Area: Cell Motility/Cytoskeleton/Structure, Protein Trafficking and Clearance, Signal Transduction
Molecular Family: Apolipoproteins, Tumor Suppressors
Antigen References: Actis Dato V and Chiabrando GA. 2018. Int J Mol Sci. E1780 Storck SE and Pietrzik CU, 2017. Pharm Res. 34(12):2637 Holtzman DM, et al. 2012, Cold Spring Harb Perspect Med. 2(3):a006312
Gene ID: 4035299858
UniProt: View information about LRP1 on UniProt.org
Clone: 11H4
Regulatory Status: RUO
Other Names: LRP1, LDL Receptor Related Protein 1, CD91, APOER
Isotype: Mouse IgG1, κ