Biolegend, 859201, Purified anti-Myelin Basic Protein Phospho (Thr98) Antibody, 25μg

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP. Phosphorylation of MBP at Thr98 has been shown to decrease its interaction with the lipid bilayer and lead to the destabilization of the compact myelin sheath.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human MBP when phosphorylated at threonine 98
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azidePrevious lot, B292474, was formulated with Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide. For further information, please contact BioLegend Technical Support or Customer Service .
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C
Application: WB - Quality tested Direct ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Yon M, et al. 1994. J NeuroImmunol. 58(2):121 (ELISA, WB)
RRID: AB_2814581 (BioLegend Cat. No. 859201)
Structure: MBP is a 304 amino acid protein with predicted molecular mass of ~33 kD. MPB exists in multiple isoforms with observed molecular masses of ~ 18, 21 and 33 kD.
Distribution: Tissue Distribution: Central and peripheral nervous system Cellular Distribution: Cytoskeleton, plasma membrane, cytosol, and extracellular
Function: Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
Interaction: MBP forms homodimer
Cell Type: Oligodendrocytes
Biology Area: Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References: Llyasov AA, et al. 2018. Front Neurosci. 12:733 Muller C, et al. 2013. Front Cell Neurosci. 7:169 Harauz G, et al. 2013. J Neurochem. 125(3):334
Gene ID: 4155
UniProt: View information about Myelin Basic Protein Phospho Thr98 on UniProt.org
Clone: BL28592
Regulatory Status: RUO
Other Names: MBP, Golli-MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein
Isotype: Mouse IgG2a, κ