Biolegend, 862702, Purified anti-RBFOX1 (A2BP1) Antibody, 100μg

Rbfox1 is a member of the RNA binding protein family that shares a conservative RNA recognition motif. Rbfox1 has been shown to modulate alternative splicing by binding the consensus RNA sequence motif (U)GCAUG within introns. The interaction between cytoplasmic Rbfox1 and mRNA affects mRNA stability and translation. The binding of Rbfox1 protein to the C-terminus of ataxin-2 may be a contributing factor to the development of spinocerebellar ataxia type 2 .
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2801247 (BioLegend Cat. No. 862701) AB_2801248 (BioLegend Cat. No. 862702)
Structure: RBFOX1 is a 416 amino acid protein with an apparent molecular mass of ~ 45 kD.
Distribution: Tissue Distribution: brain and heart. Cellular Distribution: nucleus, golgi apparatus, and cytosol.
Function: RBFOX1 is an RNA binding protein that modulates mRNA alternative splicing.
Interaction: RNA, ataxin-2
Cell Type: Neurons, Oligodendrocytes
Biology Area: Neurodegeneration, Protein Synthesis, Transcription Factors
Antigen References: Wang ET, et al. 2016. J. Neurosci. 36(45):11418 Duan W, et al. 2016. Mol Neurobiol. 53(3):1637 Kuroyanagi H, 2009. Cell Mol Life Sci. 66(24):3895
Gene ID: 54715268859302920
UniProt: View information about RBFOX1 on UniProt.org
Clone: A18069B
Regulatory Status: RUO
Other Names: RBFox1, A2BP1, RNA Binding fox-1 Homolog 1, Hexaribonucleotide Binding Protein 1, FOX1
Isotype: Mouse IgG2b, κ