Biolegend, 865301, Purified anti-Cofilin 1 Antibody, 25μg

Cofilin 1 is a ubiquitously expressed actin-regulating protein that binds to and depolymerizes filamentous actin (F-actin), as well as inhibits G-actin polymerization in a pH-dependent mechanism. Cofilin 1 plays a major role in cell migration, particularly tumor cell migration and invasion. A recent study using colorectal cancer as model, compared expression of cofilin 1 in cancer tissue and adjacent non-tumor tissue and suggested that it may be used as a novel diagnostic tumor marker in various cancers 4 .
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CFL1 recombinant protein (a.a. Met 1 - Leu 166)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For WB on brain lysates, 5 - 10 µg/mL is recommended.
RRID: AB_2810771 (BioLegend Cat. No. 865301) AB_2810772 (BioLegend Cat. No. 865302)
Structure: Cofilin 1 is a 166 amino acid protein with an apparent molecular mass of ~19 kD.
Distribution: Tissue Distribution: Immune system, gastrointestinal tract, lung, testis, and skin Cellular Distribution: Nucleus, cytosol, plasma membrane, cytoskeleton, and mitochondrion
Interaction: Cofilin 1 plays a role in cytoskeleton dynamics by regulating F-actin depolimerization
Ligand/Receptor: HSPH1, LIMK1, F-actin
Cell Type: Astrocytes, Dopaminergic Neurons, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area: Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Cell Structure, Neurodegeneration, Neuroscience, Synaptic Biology
Molecular Family: Cytoskeletal Proteins, GTPases, Scaffold Proteins
Antigen References: Kanellos G and Frame MC. 2016. J Cell Sci. 129(17):3211 Walsh KP, et al. 2014. Prion. 8(6):375 Xu P, et al. 2018. Front Plant Sci. 9:1528 Mousavi S, et al. 2018. J Gastrointest Oncol. 9(5):791
Gene ID: 10721263129271
UniProt: View information about Cofilin 1 on UniProt.org
Clone: A18040J
Regulatory Status: RUO
Other Names: Cofilin 1, CFL1, CFL
Isotype: Mouse IgG1, κ