Biolegend, 866101, Purified anti-NRAS Antibody, 25μg

NRAS is a Ras gene family member originally identified in human neuroblastoma cells. Like other members of the Ras family, NRAS has GTP/GDP binding and GTPase activity, and functions as a G-like regulatory protein that plays a role in regulating cell growth. Mutations in NRAS have been shown to transform cultured cells to tumor cells, implicating its role in tumorigeneses, as potential therapeutic target for cancers such as melanoma.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human NRAS a.a. 1-186
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Lucas CD, et al. 2022. Sci Immunol. 7:eabm4032. PubMed
RRID: AB_2810783 (BioLegend Cat. No. 866101) AB_2810784 (BioLegend Cat. No. 866102)
Structure: NRAS is a 189 amino acid protein with predicted and observed molecular mass of ~ 21 kD.
Distribution: Tissue Distribution: Ubiquitously expressed. Highly expressed in appendix, gallbladder, and gastrointestinal tract. Cellular Distribution: Plasma membrane, Golgi apparatus, cytosol, and nucleus.
Function: G-Like regulatory protein that plays a role in regulating cell growth.
Interaction: RGS14, RASSF7
Ligand/Receptor: GTP, GDP
Cell Type: Astrocytes, Endothelial cells, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Oligodendrocytes
Biology Area: Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Neuroscience, Stem Cells
Molecular Family: GTPases
Antigen References: Rocks O, et al. 2005. Science. 307:1746-52. Byrne M, Saif MW. 2019. Onco. Targets Ther. S194605 Sexton RE, et al. 2019. Semin. Cancer Biol. 54:131-7.
Gene ID: 489324605
UniProt: View information about NRAS on UniProt.org
Clone: A18072A
Regulatory Status: RUO
Other Names: NRAS Proto-Oncogene, GTPase NRas, Neuroblastoma RAS Viral Oncogene Homolog
Isotype: Mouse IgG2b, κ