Biolegend, 867001, Purified anti-FXR1 Antibody, 25μg

FXR1 is an RNA binding protein that interacts with polyribosomes, specifically with the 60S ribosome subunit. In the adult brain, FXR1 is mainly localized to the cytoplasm and the proximal dendrites of neurons. In the fetal brain, FXR1 is expressed in the nucleus of neurons. Clinically, FXR1 mutations have been identified as a major genetic contributing factor to fragile X mental retardation syndrome. Recent studies also suggest that FXR family of proteins integrate dopamine signaling and glutamatergic neurotransmission.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human FXR1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - VerifiedICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2 - 10 µg per ml. For immunohistochemistry, a concentration range of 1 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Xu XL, et al. 2011. J. Neurosci. 31(39):13705-9. (WB) Battle DJ, et al. 2007. J. Biol. Chem. 282(38):27953-9. (ICC)
RRID: AB_2810795 (BioLegend Cat. No. 867001) AB_2810796 (BioLegend Cat. No. 867002)
Structure: FXR1 is a 621 amino acid protein with predicted molecular mass of ~ 70 kD and observed molecular mass of ~ 75kD.
Distribution: Tissue Distribution: Ubiquitously expressed Cellular Distribution: Cytosol and nucleus
Function: FXR1 is an RNA binding protein involved in neurodevelopmental diseases such as autism, schizophrenia, and fragile X mental retardation.
Interaction: Polyribosome, FXR2, FMR1, CYFIP2
Cell Type: Dopaminergic Neurons, GABAergic Neurons, Neurons, Oligodendrocytes
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Signal Transduction, Synaptic Biology, Transcription Factors
Molecular Family: Postsynaptic proteins, Presynaptic proteins
Antigen References: Khlghatyan J, Beaulieu JM. 2018. Front Synaptic. Neurosci. 10:22. Bardoni B, et al. 2001. Brain Res. Bull. 56:375-82. Inoue SB, et al. 2000. J. Med. Invest. 47(3-4):101.
Gene ID: 8087
UniProt: View information about FXR1 on UniProt.org
Clone: 6BG10
Regulatory Status: RUO
Other Names: FMR1 Autosomal Homolog 1, Fragile X Mental Retardation Syndrome-Related Protein 1
Isotype: Mouse IgG2a, κ