Biolegend, 868901, Purified anti-Rab6 Antibody, 25μg

Rab proteins are small GTPases that are critical for the Golgi apparatus organization. Rab6 is the most abundant Golgi-associated Rab and is expressed in several isoforms, including Rab6aA, Rab6A’, Rab6B, and Rab6C. Rab6A and Rab6A’ are ubiquitously expressed while Rab6B is selectively expressed in neuronal cells. Rab6 isoforms are involved in intra-, early, and post-Golgi trafficking. Rab6 depletion leads to increased Golgi cisternal number and accumulation of vesicles in HeLa cells, suggesting that Rab6 affects the Golgi ribbon organization via regulation of vesicle transport.
25μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Rab6A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The antibody also detects mouse Rab6 A & B. In mouse brain lysates, besides band corresponding to Rab6, it also detects a band at ~50kD that was stronger than the isotype control.
RRID: AB_2820174 (BioLegend Cat. No. 868901) AB_2820175 (BioLegend Cat. No. 868902)
Structure: RAB6 is a 208 amino acid protein with predicted and observed molecular mass of ~ 25 kD.
Distribution: Tissue Distribution: Ubiquitously expressed Cellular Distribution: Cytosol, Golgi apparatus, plasma membrane, and endoplasmic reticulum
Function: Rab6 plays a role in retrograde protein transportation from the plasma membrane to the Golgi apparatus, and the endoplasmic reticulum.
Interaction: RAB6IP1, RAB6KIFL, BICD1, BICD2, TMF1, DYNLRB1
Cell Type: Neurons
Biology Area: Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family: Golgi Markers, Organelle Markers, Postsynaptic proteins, Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References: Liu S, et al. 2015. Int Rev Cell Mol Biol. 315:1 Heffernan LF, et al. 2014. Biochem Soc Trans. 42(5):1453 Fernandes H, et al. 2009. Biochem Soc Trans. 37:1037
Gene ID: 58705156084379363123
UniProt: View information about Rab4 on UniProt.org
Clone: A17164B
Regulatory Status: RUO
Other Names: Rab6A, Rab6B, Rab6
Isotype: Mouse IgG2b, κ