Biolegend, 870801, Purified anti-GlyT2 (SLC6A5) Antibody, 25μg

Glycine transporter 2 (GlyT2) protein is encoded by the SLC6A5 gene. It is one of the major glycine transporters that is expressed predominantly in the brain stem and spinal cord, and is frequently used as cellular marker for glycinergic neurons in the central nervous system. Mutations in the SLC6A5 gene have been clinically linked to hyperekplexia 3, which has been thought to be caused by disruption of inhibitory neuronal transmission.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to a.a. 182-199 of mouse SLC6A5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1 - 5 µg/mL. For immunocytochemistry, a concentration range of 2 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2820198 (BioLegend Cat. No. 870801) AB_2820199 (BioLegend Cat. No. 870802)
Structure: GlyT2 is a 797 amino acid protein with predicted and observed molecular mass of ~87 kD.
Distribution: Tissue Distribution: Brain Cellular Distribution: Plasma Membrane
Function: Glycine transport and recycling
Ligand/Receptor: Glycine
Cell Type: Mature Neurons, Neurons
Biology Area: Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family: Neurotransmitter Receptor Trafficking/Endocytosis, Presynaptic proteins
Antigen References: Zafra F, et al. 2017. Adv Neurobiol. 16:55. Lopez-Corcuera B, et al. 2017. Adv Neurobiol. 16:13. Vandenberg RJ, et al. 2014. Trends Pharmacol Sci. 35(8):423.
Gene ID: 9152171148
UniProt: View information about GlyT2 on UniProt.org
Clone: A18174E
Regulatory Status: RUO
Other Names: GLYT-2, NET1, HKPX3, Glycine Transporter 2
Isotype: Mouse IgG2a, κ