Biolegend, 901516, Anti-HA.11 Epitope Tag Antibody (Previously Covance catalog# MMS-101R), 25microl

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.
25microl
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Preparation: Ascites
Concentration: The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/mL.
Storage & Handling: Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application: WB - Quality tested ICC, IP - Verified FC, Purification - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is a dilution of 1:1000 – 1:5000 . For immunocytochemistry, the suggested use of this reagent is a dilution of 1:1000 - 1:2000 is suggested. For immunohistochemistry, the suggested use of this reagent is a dilution of 1:1,000 is suggested. For immunoprecipitation, the suggested use of this reagent is a dilution of 1:150 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC). *Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody. This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
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RRID: AB_2820200 (BioLegend Cat. No. 901516) AB_2565335 (BioLegend Cat. No. 901513) AB_2565336 (BioLegend Cat. No. 901514) AB_2565334 (BioLegend Cat. No. 901515)
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about HA.11 on UniProt.org
Clone: 16B12
Regulatory Status: RUO
Other Names: HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously: Covance Catalog# MMS-101R
Isotype: Mouse IgG1, κ