Biolegend, 903101, Purified anti-AU1 Epitope Tag Antibody (Previously Covance catalog# PRB-130P), 100microl

AU1 is a commonly used epitope that is tagged onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Antibodies against AU1 epitope recognize proteins containing AU1 epitope tag fused to targeted proteins and allow for specific detection in several immunochemical assays such as western blotting, ELISAs and immunostaining techniques.
100microl
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: This polyclonal antibody was raised against the peptide DTYRYI (the same epitope as the monoclonal AU1 antibody).
Formulation: Phosphate-buffered solution + 0.03% thimerosal.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: WB, IHC, ICC, ELISA, Purification
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1:1,000-30,000 • ICC: 1:200-2,000 • ELISA: 1:100-400 (coating), 1:1,000-30,000 (primary)
Application Notes: This antibody is effective in immunoblotting (WB), immunohistochemistry (ICC), immunocytochemistry and ELISA.This antibody may be preferred over the monoclonal AU1 for experiments in murine systems or in experiments involving two proteins labeled with two different tags. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence.This product may contain other non-IgG subtypes.
Product Citations: Kim GB et al. 2019. Cell. 179(1):251-267 . PubMed Bodur C, et al. 2018. EMBO J. 37:19. PubMed
RRID: AB_2565028 (BioLegend Cat. No. 903101)
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about AU1 on UniProt.org
Clone: Poly29031
Regulatory Status: RUO
Other Names: AU1 epitope tag, DTYRYI epitope tag, DTYRYI tag
Previously: Covance Catalog# PRB-130P
Isotype: Rabbit Polyclonal IgG