Biolegend, 903401, Purified anti-β-Tubulin Antibody (Previously Covance catalog# MMS-410P), 250microl

Tubulin is ubiquitously expressed and is the major constituent of microtubules, which are composed of alpha and beta tubulin heterodimers. There are five distinct forms of tubulin: alpha, beta, gamma, delta, and epsilon. Beta tubulin has multiple isoforms that are expressed in mammalian tissues and localize to different places in the cell.
250microl
Verified Reactivity: Human, Mouse, Rat, Non-Human Primate, Hamster, Drosophila
Reported Reactivity: Other species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was generated against taxol-assembled microtubules.
Formulation: Phosphate-buffered solution + 0.03% Thimerosal.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: WB, ICC, IHC
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1 µg/mL (1:1000) • IF: 1,000
Application Notes: Reactivity to Drosophila was only verified with the purified format. This antibody is effective in immunoblotting and immunostaining. *Predicted MW = 55 kD The TU27 clone is broadly reactive to b-tubulin subtypes and binds outside of the C-terminal isotype domains, but does not cross react with alpha-tubulin by immunoblotting. It recognizes a highly conserved epitope and reacts with ß-tubulin found in vertebrate as well as invertebrate tissues.
Additional Product Notes: For Western blotting, the suggested use of this reagent is 1.0 - 10 µg/mL in Drosophila and 1.0 µg/mL in other species.
Application References(PubMed link indicates BioLegend citation): MK Lee, et al. 1990. Cell Motil. Cytoskel. 17:118. JE Alexander, et al. 1991. Proc Natl Acad Sci USA. 88:4685. J Lisztwan, et al. 1998. EMBO J. 17:368. Fujita T, et al. 2015. PLoS One. 10:e0116579. (WB) PubMed Ai M, et al. 2013. J. Neurosci. 33:10741. (WB) PubMed
Product Citations: Venkatesan A, et al. 2019. Int J Mol Sci. 20:4. PubMed Lence T, et al. 2016. Nature. 540:242-247. PubMed Mitra S, et al. 2021. Dev Biol. 478:205. PubMed Heine D, et al. 2016. Mycologia. 108: 581 - 589. PubMed Courgeon M, et al. 2018. J Cell Sci. 131:jcs220251. PubMed Banerjee SJ, et al. 2021. PLoS One. 16:e0256738. PubMed Ho CH, et al. 2022. EMBO Rep. 23:e53231. PubMed Ai M, et al. 2013. J Neurosci. 33:10741-10749. PubMed Fujita T, et al. 2015. PLoS One. 10:e0116579. PubMed Astigarraga S, et al. 2018. Development. 145:. PubMed Swiatlowska P, et al. 2020. Nanoscale. 12:16315. PubMed de Jesus AA, et al. 2019. J Allergy Clin Immunol. 143:1939. PubMed Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed Sah M, et al. 2020. Neurobiology of Disease. 78.45694444. PubMed Vernia S et al. 2016. eLife. 5 pii: e17672. PubMed Bawankar P, et al. 2021. Nat Commun. 12:3778. PubMed Knuckles P, et al. 2018. Genes Dev. 29:13. PubMed Church TW, et al. 2021. Elife. 10:. PubMed Kwon OS, et al. 2021. Nat Commun. 12:1351. PubMed Wan J, et al. 2016. Brain. 139: 2877 - 2890. PubMed
RRID: AB_2565030 (BioLegend Cat. No. 903401)
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microtubules
Gene ID: 533307
UniProt: View information about beta-Tubulin on UniProt.org
Clone: TU27/Tubulin
Regulatory Status: RUO
Other Names: Tubulin, beta 2A
Previously: Covance Catalog# MMS-410P
Isotype: Mouse IgG1, κ