Biolegend, 903702, Purified anti-CD243/ABCB4 (MDR-1, MDR3) Antibody (Previously Covance catalog# SIG-38710), 25microl

Clone C219 recognizes an internal, highly conserved amino acid sequence (VQEALD & VQAALD) found in both MDR1 and MDR3 isoforms of P-glycoprotein. The specific binding of the clone C219 was stronger in epitope sequence VQAALD (N-Terminal fragment) than the epitope sequence VQEALD (C-terminal fragmant) (Georges E., et al., 1990). Analysis of p-glycoprotein expression in skeletal and cardiac muscle revealed that C219 cross reacts with a 200kDa protein that migrates in the same position as myosin. C219 has also been reported to detect the 185kDa c-erbB2 protein (Liu, et al., 1997). Care should be taken in the interpretation of true positive P-glycoprotein expression when using myosin-containing tissues or tissues known to have p185 c-erbB2 expression.
25microl
Verified Reactivity: Human, Mouse
Reported Reactivity: Rat, Dog, Non-Human Primate
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was made against SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.
Formulation: Phosphate-buffered solution with BSA + 0.09% NaN3.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Store between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified IHC-F, ICC, FC, WB, ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:20 - 1:40 is suggested. For western blotting, the suggested use of this reagent is a 1:100 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Hisano Y, et al. 2011. J. Biol. Chem. 286: 1758. (WB) Beck WT, et al. 1996. Cancer Res. 56:3010. (IHC-P, IHC-F, ICC, FC) PubMed Evans CH, Baker PD. 1992. Cancer Res. 52: 5893. (WB, FC) Toth K, et al. 1992. Am J Pathol. 140:1009. (IHC-P, ICC, WB) Finstad CL, et al. 1991. J Histochem Cytochem. 39:1603. (FC, WB, IHC-P Georges E, et al. 1990. PNAS. 87:152. (ELISA, IHC-P) Duan Z, et al. 2014. BMC Cancer. 14:681. (WB) Inui N, et al. 2012. J Gerontol A Biol Sci Med Sci. 67:1049. (FC, IF) Erickson MA, et al. 2012. J Neuroinflammation. 9:150. (WB) Rao VV, et al. 1994. Cancer Res. 54:1536. (WB) PubMed Lee JJ, et al. 1996. Cancer. 77:1892. (IHC) PubMed
Product Citations: Lee J, et al. 1996. Cancer. 77:1892-1898. PubMed Giddings EL, et al. 2021. Nat Commun. 12:2804. PubMed Afrouzian M, et al. 2018. Biochem Pharmacol. 156:467. PubMed Sophie Kang S, et al. 2016. PLoS One. 11:e0165638. PubMed Beck W, et al. 1996. Cancer Res. 56:3010-3020. PubMed Song YK, et al. 2019. J Pharmacol Exp Ther. 368:79. PubMed
RRID: AB_2565033 (BioLegend Cat. No. 903701)
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Gene ID: 5244
UniProt: View information about CD243/ABCB4 on UniProt.org
Clone: C219
Regulatory Status: RUO
Other Names: Multidrug resistance protein 3, P-glycoprotein 3, multiple drug resistance 3, ATP-binding cassette sub-family B member 4, P glycoprotein 3/multiple drug resistance 3
Previously: Signet Catalog# 8710-01Covance Catalog# SIG-38710
Isotype: Mouse IgG1, κ