Biolegend, 906102, Purified anti-6-His Epitope Tag Antibody (Previously Covance catalog# MMS-156P), 500microl

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).
500microl
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Formulation: Phosphate-buffered solution + 0.03% Thimerosal.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: Store between 2°C and 8 °C.
Application: WB - Quality tested ICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000 dilution. For immunocytochemistry, the suggested use is 1:1000 – 1:4000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.
Application References(PubMed link indicates BioLegend citation): Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB) Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (IF) PubMed Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB) Bartsch D, et al. 1998. Cell. 95:211. (IF) Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed
Product Citations: Dotiwala F, et al. 2016. Nat Med. 22:210-216. PubMed Xia T, et al. 2023. PLoS Pathog. 19:e1011232. PubMed Gregg BM, et al. 2024. MBio. e0310623:15. PubMed Montegna E, et al. 2012. PLoS One. 7:e31156. PubMed Huang L, et al. 2013. Nat Biotechnol. 31:350-356. PubMed Marshall RS, et al. 2020. J Cell Sci. 133:jcs249862. PubMed Marques PE et al. 2019. Dev Cell. 50(3):283-295 . PubMed Sánchez-Bellver L, et al. 2022. Int J Mol Sci. 23:. PubMed Mukherjee S et al. 2019. Developmental cell. 49(1):63-76 . PubMed Fuentes E, et al. 2003. J Biol Chem. 278:21188-21196. PubMed Marshall RS, et al. 2020. J Cell Sci. :133. PubMed Kojima H, et al. 2001. J Biol Chem. 276:19238-19243. PubMed
RRID: AB_2734683 (BioLegend Cat. No. 906113) AB_2565061 (BioLegend Cat. No. 906101) AB_2565062 (BioLegend Cat. No. 906102)
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about 6-His on UniProt.org
Clone: 6-His
Regulatory Status: RUO
Other Names: polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Previously: Covance Catalog# MMS-156P
Isotype: Mouse IgG1, κ