Biolegend, 907002, Purified anti-CD243 (MDR-1) Antibody (Previously Covance catalog# SIG-38720), 25microl

CD243 (MDR-1) belongs to the ATP binding cassette (ABC) transporter family. With an approximate molecular mass of 170 kD, it consists of two homologous halves. Each half contains two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs). The TMDs span the membrane six times, forming a chamber with a 12 transmembrane α-helix structure. NBDs drive the transport process through ATP coupling and hydrolysis and are located at the cytoplasmic face of the membrane. CD243 transports various molecules across cellular membranes and is involved in multidrug resistance. MDR-1 is expressed on hematopoietic stem cells, T cells, B cells, and NK cells as well as on many multidrug resistant neoplastic cells. CD243 interacts with Caveolin, RING finger protein 1B, AAP1, p53, Orphan nuclear receptor PX, and cytochrome P450.
25microl
Verified Reactivity: Human
Reported Reactivity: Non-Human Primate
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution with BSA + 0.09% NaN3.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by affinity chromatography.
Storage & Handling: Store between 2°C and 8°C.
Application: IHC-P, ICC, IP FC, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining. The optimal working dilution should be determined for each specific assay condition. • IHC: ≥1:20 for Biotin based detection systems such as USA Ultrastreptavidin Detection (Cat. No. 929501) Tissue Sections: Formalin-fixed, paraffin-embedded tissues. Pretreatment: Not Required. Incuabation: 20-60 minutes at room temperature.
Application Notes: This antibody is effective in immunoblotting, immunohistochemistry (IHC), immunocytochemistry (ICC) and immunopurification (IP). Positive Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Positive Tissues: Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretaory epithelium). C494 detects a gene-specific, internal cellular epitope (PNTLEGN) present only on the Mdr1 isoform of P-glycoprotein. C494 does not cross react with Mdr3. C494 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant 130kDa mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections. Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein expression. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.
Application References(PubMed link indicates BioLegend citation): Georges E, et al.1990. PNAS. 87:152. (IHC, ELISA) Toth K, et al.1992. Am. J. Pathol. 140:1009. (IHC,ICC,WB,ELISA) Rao VV, et al. 1994. Cancer Res. 54:1536. (WB) PubMed Lee JJ, et al. 1996. Cancer. 77:1892. (IHC) PubMed Beck WT, et al. 1996. Cancer Res. 56:3010. (IHC, ICC, FC) PubMed Villar VH, et al. 2012. PLos One. 7:37735. (IHC) PubMed Mahadevan D, et al. 2013. Cancer. 119:371. (IHC) PubMed Martin-Broto J, et al. 2014. Mol Cancer Ther. 13:249. (IHC) PubMed
Product Citations: Lee J, et al. 1996. Cancer. 77:1892-1898. PubMed Mahadevan D, et al. 2013. Cancer. 119:371-379. PubMed Martin-Broto J, et al. 2014. Mol Cancer Ther. 13:249-259. PubMed Beck W, et al. 1996. Cancer Res. 56:3010-3020. PubMed Villar V, et al. 2012. PLoS One. 7:e37735. PubMed
RRID: AB_2565071 (BioLegend Cat. No. 907001)
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Gene ID: 5243
UniProt: View information about CD243 on UniProt.org
Clone: C494
Regulatory Status: RUO
Other Names: P-glycoprotein 1, ABC20, CLCS, GP170, MDR1, P-GP, PGY1, colchicin sensitivity, doxorubicin resistance, multidrug resistance protein 1, ATP-binding cassette, sub-family B (MDR/TAP), member 1, MRP1, Multidrug resistance protein 1
Previously: Signet Catalog# 8720-01Covance Catalog# SIG-38720
Isotype: Mouse IgG2a, κ