Biolegend, 909101, Purified anti-Tubulin-β 2B (TUBB2B) Antibody (Previously Covance catalog# MMS-422P), 200microl

Tubulin is a major component of microtubules. It exists as a heterodimer consiting of an alpha and a beta subunit. Class II beta tubulin is the major form of tubulin beta in neurons, although it is not neuronal specific and is found in many other tissues including lung tissue and Schwann cells.
200microl
Verified Reactivity: Human, Mouse, Rat, Frog
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against the synthetic peptide CEEEEGEDEA found at the c-terminus of beta II tubulin.
Formulation: Phosphate-buffered solution + 0.03% Thimerosal.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.4 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunoblotting (WB) and immunocytochemistry (ICC). Xenopus reactivity was verified by Dr. Daniel Levy’s lab at the University of Wyoming
Application References(PubMed link indicates BioLegend citation): Moody SA, et al. 1996. J. Comp. Neuro. 364: 219. Dozier JH, et al. 2003. Breast Cancer Res. 5:R157.
Product Citations: Jevtic P, et al. 2019. PLoS One. 22:e0215740. PubMed
RRID: AB_2565093 (BioLegend Cat. No. 909101)
Structure: Expected MW: 50 kD
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microtubules
Gene ID: 347733
UniProt: View information about Tubulin-beta 2B on UniProt.org
Clone: 7B9
Regulatory Status: RUO
Other Names: Tubulin beta-2B chain, class IIb beta-tubulin, class II beta-tubulin isotype, tubulin, beta polypeptide paralog
Previously: Covance Catalog# MMS-422P
Isotype: Mouse IgG3