Biolegend, 915106, Purified anti-BRST-2 (GCDFP-15) Antibody, 100μg

The BRST-2 antigen is expressed by apocrine sweat glands, eccrine glands (variable), minor salivary glands, bronchial glands, metaplasmic epithelium of the breast, benign sweat gland tumors of the skin and the serous cells of the submandibular gland. Breast carcinomas (primary and metastatic lesions) with apocrine features express the BRST-2 antigen.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was generated against Gross Cystic Disease Fluid Protein 15.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The BRST-2 clone D6 recognizes a 15 kD glycoprotein known as Gross Cystic Disease Fluid Protein-15 (GCDFP-15). Antigen retrieval is not required. The BRST-2 epitope is heat-sensitive; paraffin sections that have been heat fixed at 60°C may lose antibody binding sites.
Application References(PubMed link indicates BioLegend citation): Yoshimura Y, et al. 2011. Jpn. J. Clin. Oncol. 41:358. (IHC) PubMed Fan CY, et al. 2000. Am. J. Surg. Pathol. 24(4):579. PubMed Wick MR, et al. 1989. Hum. Pathol. 20(3):281. PubMed Mazoujian G, et al. 1984. Am. J. Surg. Pathol. 8(1):43. PubMed Mazoujian G, et al. 1983. Am. J. Pathol. 110:105. PubMed Brown RW, et al. 1997. Am. J. Clin. Path. 107:12. PubMed
RRID: AB_2616964 (BioLegend Cat. No. 915106)
Structure: BRST-2 (GCDFP-15) is a 17 kD protein.
Distribution: Extracellular and nucleus.
Function: Diseases associated with BRST-2 include florid papillomatosis of the nipple and breast signet ring cell adenocarcinoma. Interacts with AZGP1.
Biology Area: Cancer Biomarkers, Cell Biology
Gene ID: 5304
UniProt: View information about BRST-2 on UniProt.org
Clone: D6
Regulatory Status: RUO
Other Names: Prolactin-inducible protein (PIP), secretory actin-binding protein, gross cystic disease fluid protein 15, BRST2, GCDFP15, GCDFP-15, GPIP4
Isotype: Mouse IgG2a, κ