Biolegend, 916504, Purified anti-Cytokeratin, HMW Antibody, 100μg

This antibody is specific for high molecular weight cytokeratins 1, 5, 10 and 14. Clone 34βE12 is immunoreactive with squamous and adeno-squamous carcinomas. In normal epithelia, the antibody stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This antibody is also useful in differentiating benign and malignant tumors of the prostate gland.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was developed using a solubilized keratin extracted from human stratum corneum.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality testedWB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 μg/ml is suggested. For Western blotting, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The Cytokeratin, High Molecular Weight (HMW) antibody (clone 34ßE12) reacts primarily with complex epithelium such as squamous, ductal and transitional epithelium and corresponding tumors.
Application References(PubMed link indicates BioLegend citation): Gown AM, Vogel AM. 1985. Am. J. Clin. Pathol. 84:413. (IHC) Gown AM, Vogel AM. 1982. J. Cell Biol. 95:414. (IHC)
RRID: AB_2629548 (BioLegend Cat. No. 916504)
Distribution: Tissue distribution: skin, prostate or squamous cell carcinoma. Cellular distribution: cytoplasm.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Gene ID: NA
UniProt: View information about Cytokeratin on UniProt.org
Clone: 34βE12
Regulatory Status: RUO
Other Names: Cytokeratin, high-molecular weight keratin (HMWK), high-molecular weight cytokeratin (HMWCK)
Isotype: Mouse IgG1, κ