Biolegend, 916605, Purified anti-Podoplanin (Lymphatic Endothelial Marker) Antibody, 25μg

Podoplanin is expressed by a variety of normal cells, which include breast and prostate myopepithelial cells, follicular dendritic cells, basal keratinocytes of the skin and cervix (focal), type I pneumocytes, ependymal cells, and fetal cerebral germinal matrix cells. Podoplanin is a marker of lymphatic differentiation because it is expressed by normal human lymphatic endothelium, but not by vascular endothelium. It is diagnostically a useful marker for the evaluation of a variety of neoplasms, including Kaposi sarcoma mesothelioma, testicular germ cell tumors, and cutanneous sebaceous neoplasms.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: M2A protein derived from human germ cell tumors.
Formulation: Phosphate-buffered solution, pH 7.2.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB, ICFC, ICC - Verified ELISA Detection - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 μg/mL is suggested. For Western blotting, the suggested use of this reagent is 2.0 - 5.0 µg per mL. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: ELISA Detection5 and immunocytochemistry4.
Application References(PubMed link indicates BioLegend citation): Hamanaka T, et al. 2011. Invest. Ophthalmol. Vis. Sci. 52:8849. (IHC-P) Choi WW, et al. 2005. Mod. Pathol. 18:143. (IHC-P) Chu AY, et al. 2005. Mod. Pathol. 18:105. (IHC-P) PubMed Yoon C, et al. 2008. Blood 112:1129. (ICC) PubMed Schacht V, et al. 2005. American Journal of Pathology 166:913. (ELISA Detection, WB) PubMed Marks A, et al. 1999. British Journal of Cancer 80:569.
Product Citations: Burlingame EA, et al. 2021. Cell Rep Methods. 1:. PubMed Yoon C, et al. 2008. Blood. 112:1129-1138. PubMed Francescone R, et al. 2020. Cancer Discovery. 11(2):446-479. PubMed Schacht V, et al. 2005. Am J Pathol. 166:913-921. PubMed Chu A, et al. 2005. Mod Pathol. 18:105-110. PubMed
RRID: AB_2565819 (BioLegend Cat. No. 916605) AB_2565820 (BioLegend Cat. No. 916606)
Structure: 35 kD.
Distribution: Membrane and plasma membrane.
Function: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels.
Interaction: Cytoskeletal signaling.
Biology Area: Cancer Biomarkers, Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Gene ID: 10630
UniProt: View information about Podoplanin on UniProt.org
Clone: D2-40
Regulatory Status: RUO
Other Names: Podoplanin, T1-alpha, hT1alpha-1, hT1alpha-2, PA2.26 antigen, glycoprotein 36, lung type-I cell membrane-associated glycoprotein (T1A-2)
Isotype: Mouse IgG1, κ