Biolegend, 916611, Alexa Fluor® 488 anti-Podoplanin (Lymphatic Endothelial Marker) Antibody, 25tests

Podoplanin is expressed by a variety of normal cells, which include breast and prostate myopepithelial cells, follicular dendritic cells, basal keratinocytes of the skin and cervix (focal), type I pneumocytes, ependymal cells, and fetal cerebral germinal matrix cells. Podoplanin is a marker of lymphatic differentiation because it is expressed by normal human lymphatic endothelium, but not by vascular endothelium. It is diagnostically a useful marker for the evaluation of a variety of neoplasms, including Kaposi sarcoma mesothelioma, testicular germ cell tumors, and cutanneous sebaceous neoplasms.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: M2A protein derived from human germ cell tumors.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Additional Product Notes: For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803).
Application References(PubMed link indicates BioLegend citation): Hamanaka T, et al. 2011. Invest. Ophthalmol. Vis. Sci. 52:8849. (IHC-P) Choi WW, et al. 2005. Mod. Pathol. 18:143. (IHC-P) Chu AY, et al. 2005. Mod. Pathol. 18:105. (IHC-P) PubMed Yoon C, et al. 2008. Blood 112:1129. (ICC) PubMed Schacht V, et al. 2005. American Journal of Pathology 166:913. (ELISA Detection, WB) PubMed Marks A, et al. 1999. British Journal of Cancer 80:569.
RRID: AB_3720619 (BioLegend Cat. No. 916611) AB_3720619 (BioLegend Cat. No. 916612)
Structure: 35 kD
Distribution: Membrane and plasma membrane
Function: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels.
Interaction: Cytoskeletal signaling
Biology Area: Cancer Biomarkers, Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Gene ID: 10630
UniProt: View information about Podoplanin on UniProt.org
Clone: D2-40
Regulatory Status: RUO
Other Names: Podoplanin, T1-alpha, hT1alpha-1, hT1alpha-2, PA2.26 antigen, glycoprotein 36, lung type-I cell membrane-associated glycoprotein (T1A-2)
Isotype: Mouse IgG1, κ