Biolegend, 931701, c-Myc Peptide Tag (Previously Covance catalog# PEP-150P), 1mg

The c-myc tag is often used as a protein modification in order to simplify the labeling and detection of proteins. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to targeted proteins and allow for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.
1mg
Purity: >90% pure by HPLC
Formulation: Lyophilized.
Storage & Handling: Store lyophilized or at -20°C in solution
Application: IP, WB
Recommended Usage: Soluble in PBS. The optimal working dilution should be determined for each specific assay condition. • Elution of epitope tag fusion proteins from immunoaffinity columns: 400 ug/mL • Competitive inhibition of antibody in western or immunostaining: 10-fold molar excess over optimized (low) antibody
Application Notes: This synthetic peptide (H2N-CEQKLISEEDL-COOH) corresponds to the epitope detected by the 9E10 monoclonal antibody. This peptide can be used in competition experiments, or to elute bound c-myc fusion proteins from the 9E10 affinity matrix.
Application References(PubMed link indicates BioLegend citation): Enquist-Newman M, et al. 2001. Mol. Biol. Cell. 12: 2601. Ferrando A, et al. 2001. Nucleic Acids Res. 29:3685.
Biology Area: Cell Biology
Gene ID: 4609
UniProt: View information about c-Myc on UniProt.org
Regulatory Status: RUO
Other Names: (CEQKLISEEDL), Myc Proto-Oncogene Protein, Proto-Oncogene C-Myc, Transcription Factor P64, Class E Basic Helix-Loop-Helix Protein 39, Avian Myelocytomatosis Viral Oncogene Homolog, V-Myc, Myc-Related Translation/Localization
Previously: Covance Catalog# PEP-150P