Biolegend, 935301, Purified anti-c-Myb Antibody, 25μg

c-Myb is a proto-oncogene protein that functions as a transcription factor and a key regulator of cell proliferation and differentiation. As a DNA binding protein, it plays a role in regulating cell cycle and gene expression. c-Myb expression is elevated in hematopoietic progenitor cells and decreases following cell differentiation. Increased or aberrant expression of c-Myb as well as duplication and c-terminal deletion have been reported in various malignancies suggesting it plays a critical role in tumorigenesis.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant protein of human MYB corresponding to amino acid residues 391-640
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is predicted to recognize isoforms 1, 2, 4, 7, and 8 based off complete sequence homology of the immunizing peptide and the corresponding region in each isoform.ICC test failed. The clone incorrectly stains the negative control in the cytosol.
RRID: AB_2832892 (BioLegend Cat. No. 935301) AB_2832893 (BioLegend Cat. No. 935302)
Structure: Myb is a 640 amino acid protein with a predicted molecular weight of 72 kD.
Distribution: Nucleus/differential expression
Function: Gene transcription, cell proliferation and differentiation
Cell Type: Epithelial cells, Leukemia
Biology Area: Cell Biology, Stem Cells, Transcription Factors
Antigen References: Schulz C, et al. 2012. Science. 336(6077):86-90. Martin C, et al. 1997. Trends Genet. 13(2):67-73. Mucenski M, et al. 1991. Cell. 65(4):677-89.
Gene ID: 4602
UniProt: View information about c-Myb on UniProt.org
Clone: W16209A
Regulatory Status: RUO
Other Names: Myb, c-Myb, Transcriptional activator Myb, Proto-oncogene c-Myb, MYB Proto-Oncogene, Transcription Factor
Isotype: Rat IgG2a, κ