Biolegend, 936106, Alexa Fluor® 647 anti-TFEB Antibody, 100tests

The autophagy-lysosomal pathway is required for the quality control degradation of proteins and other macromolecules. The transcription factor EB (TFEB) plays a critical role in inducing expression of genes involved in autophagy and lysosomal biogenesis. The subcellular localization and activity of TFEB are regulated by mechanistic target of rapamycin (mTOR)-mediated phosphorylation. Under nutrient-replete conditions, mTORC1 phosphorylates TFEB promoting its cytosolic retention by 14-3-3 proteins and nuclear export through CRM1. Under nutrient deprivation, TFEB phosphorylation decreases, promoting its nuclear import and transactivation function. TFEB is frequently dysregulated in cancer, and is also involved in the clearance of intracellular pathogenic targets in a variety of diseases, such as Parkinson's and Alzheimer's, suggesting that novel therapeutic strategies could be based on the modulation of TFEB activity.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human TFEB protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: When TFEB is phosphorylated, a molecular weight increase may be observed by western blot (Martina JA, et al. 2013. J Cell Biol. 200:475). Differences in the observed molecular weight across cell lines and tissues may be attributed to differences in basal TFEB phosphorylation in these samples.
RRID: AB_2936767 (BioLegend Cat. No. 936105) AB_2936767 (BioLegend Cat. No. 936106)
Structure: TFEB is a 476 amino acid protein with a predicted molecular weight of 53 kD.
Distribution: Nucleo-cytoplasmic/Ubiquitously expressed
Function: Transcription Factor required for inducing expression of genes involved in autophagy and lysosomal biogenesis.
Biology Area: Cell Biology, Transcription Factors
Antigen References: Napolitano G, et al. 2018. Nat Commun. 9:3312. Peña-Llopis S, et al. 2011. EMBO J. 30:3242. David R, et al. 2011. Nat Rev Mol Cell Biol. 12:404. Settembre C, et al. 2011. Science. 332:1429.
Gene ID: 7942
UniProt: View information about TFEB on UniProt.org
Clone: W18197C
Regulatory Status: RUO
Other Names: Transcription Factor EB
Isotype: Rat IgG2a, κ