Biolegend, 936201, Purified anti-Aurora B Recombinant Antibody, 25μg

Aurora B, also known as AURKB, is a member of the Aurora family of Serine/Threonine protein kinases. The expression level of Aurora B is dramatically increased during the S/G2/M phases of the cell cycle. Aurora B plays a critical role in cell division and chromosome-microtubule interactions during mitosis. Aurora B forms the chromosomal passenger complex (CPC) together with INCENP, Survivin, and Borealin and functions to ensure faithful chromosome segregation. The CPC localizes to the kinetochores from prophase to metaphase and migrates to the central spindle and the midbody during telophase. Aurora B phosphorylates Histone H3 at residues Serine 53 and Serine 28 during prophase, leading to the dissociation of HP1 from chromatin and condensation of chromosome. Aurora B was found to be overexpressed in several malignant cancers and is involved in promoting tumor development and progression.
25μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Rat
Immunogen: Partial recombinant human Aurora B protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not recognize mouse Aurora B. This recombinant antibody clone is derived from clone W16153A.
RRID: AB_2832908 (BioLegend Cat. No. 936201) AB_2832909 (BioLegend Cat. No. 936202)
Structure: 344 amino acids with a predicted molecular weight of 39.3 kD. Contains a Serine/Threonine kinase domain.
Distribution: Nucleus, chromosome, mitotic spindle, midbody
Function: Aurora B is a Serine/Threonine protein kinase belonging to the Aurora kinase family. It plays an important role in cell division by regulating mitosis and cytokinesis.
Interaction: Forms the chromosomal passenger complex (CPC) with INCENP, Survivin, and Borealin. Interacts with RACGAP1, CDCA1, EVI5, JTB, NDC80, PSMA3, SEPT1, SIRT2, TACC1, SPDYC, p53/TP53, NOC2L, TTC28, and RNF2/RING1B.
Biology Area: Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Abe Y, et al. 2016. Dev Cell. 36:487. Ritter A, et al. 2015. Cell Cycle. 14:3755. Park H, et al. 2014. Cell Cycle. 13:2391. Hegyi K & Méhes G. 2012. Pathol Oncol Res. 18:761. Carmena M, et al. 2009. Curr Opin Cell Biol. 21:796. Shannon KB, et al. 2002. Curr Biol. 12:R458.
Gene ID: 9212
UniProt: View information about Aurora B on UniProt.org
Clone: QA18A11
Regulatory Status: RUO
Other Names: Aurora kinase B, AURKB, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, Aurora/IPL1-related kinase 2 (ARK-2), STK-1, Protein Phosphatase 1, Regulatory Subunit 48, Serine/Threonine-Protein Kinase Aurora-B, Serine/Threonine-Protein
Isotype: Rat IgG2a, κ