Biolegend, 936902, Purified anti-STAT5 Phospho (Tyr694) Antibody, 100μg

STAT5 is a member of the signal transducer and activator of transcription factors (STAT) family, mediating growth and cytokine signaling. STAT5 consists of two closely related family members, STAT5A and STAT5B, which exhibit 96% sequence homology and are functionally redundant. Upon activation, STAT5 is phosphorylated by receptor tyrosine kinases and, in turn, forms homodimers or hererodimers with other family members through its SH2 domains. The dimerized STAT5 translocates to the nucleus and binds to the STAT5 response element (TTCXXXGAA). STAT5 plays crucial regulatory roles in cell proliferation, anti-apoptosis, early B cell differentiation, and T cell development.
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Peptide from Human Stat5A phosphorylated at Tyr694
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This recombinant antibody clone is derived from clone A17016B. No changes or modifications were done to the antibody sequence or scaffold.
Product Citations: Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
RRID: AB_2861121 (BioLegend Cat. No. 936902)
Structure: 794 amino acids, predicted molecular weight of 91 kD; contains a SH2 domains responsible for homodimerization or heterodimerization.
Distribution: Cytoplasm; phosphorylated form translocates to the nucleus.
Function: STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin.
Antigen References: Bell BD, et al. 2013. Nat. Immunol. 14:364. Cholez E, et al. 2012. Leukemia. 26:2390. Heltemes-Harris LM, et al. 2012. Curr. Opin. Immunol. 24:146. Heltemes-Harris LM, et al. 2011. Ann. N Y Acad. Sci. 1217:18. Malin S, et al. 2010. Curr. Opin. Immunol. 22:168. Xu J, et al. 2009. J. Neurosci. 29:2022.
Gene ID: 6776
UniProt: View information about STAT5 Phospho Tyr694 on UniProt.org
Clone: A17016B.Rec
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 5A
Isotype: Mouse IgG1, κ