Biolegend, 938701, Purified anti-SARS-CoV-2 S Protein S1 Recombinant Antibody, 25μg

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single- stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 is responsible for binding to the host cell via ACE2 receptor and the S2 subunit is responsible for fusion with the host cell. This antibody is specific for the S1 subunit of SARS-CoV-2 and was derived, sequenced and expressed from patients that had recovered from COVID-19 infection.
25μg
Verified Reactivity: SARS-CoV-2
Antibody Type: Recombinant
Host Species: Human
Immunogen: SARS-CoV-2
Formulation: 140mM Hepes, pH 7.5, 70 mM NaCl,, 32 mM NaOAc, 0.035% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Block - Quality tested ELISA - Verified
Recommended Usage: Each lot is quality control tested by its ability to block the binding between recombinant human ACE2 (Cat. No. 792002) and SARS-CoV-2 S protein S1-Fc chimera (Cat. No. 793004). ND50 range is 0.03 - 0.12 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application. Recommended concentration for direct ELISA application is 45 ng/mL.
Application Notes: Note: Clone AM001414 is also referred to as Clone 414-1
Additional Product Notes: Prior to use, quick spin the antibody vial to ensure recovery of maximum volume. Antibody can be stored in single use aliquots in -20°C for 2 years. Avoid repeated freeze and thaws.
Application References(PubMed link indicates BioLegend citation): Wan J. et al. 2020. bioRxiv. doi: https://doi.org/10.1101/2020.05.19.104117
Product Citations: Fischer K, et al. 2024. Nat Biotechnol. . PubMed Connelly GG, et al. 2022. Cell Rep Methods. 2:100273. PubMed
RRID: AB_2876764 (BioLegend Cat. No. 938701) AB_2876764 (BioLegend Cat. No. 938702)
Structure: Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.
Distribution: Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
Function: Host cell surface receptor binding
Interaction: ACE2
Ligand/Receptor: ACE2
Biology Area: COVID-19
Antigen References: Walls AC, et al. 2020. Cell. 181(2):281-292. Yan R, et al. 2020. Science. 367 (6485):1444-1448. Wrapp D, et al. 2020. Science. 367 (6483):1260-1263. Shang J, et al. 2020. PNAS. 117(21):11727-11734
Gene ID: 43740568
UniProt: View information about Viral Protein on UniProt.org
Clone: AM001414
Regulatory Status: RUO
Other Names: Spike glycoprotein, S glycoprotein (E2)
Isotype: Human IgG1, κ