Biolegend, 939602, Purified anti-PDPK1 Phospho (Ser241) Antibody, 100μg

3-Phosphoinositide-Dependent Protein Kinase 1 (PDPK1) is a serine/threonine kinase that plays a central role in diverse cellular pathways, and can function as a critical activator of several prosurvival and oncogenic protein kinases. PDPK1 functions by phosphorylating and activating members of the AGC family of serine kinases, including Akt1, protein kinases A and C, and p70 ribosomal protein S6 kinase. Loss or reduction of PDPK1 in the thymus results in defective T-cell differentiation and proliferative expansion. Serine 241 of PDPK1 is an autophosphorylation site located within the activation loop of the protein and is required for full enzymatic function. This modification is considered a candidate pharmacodynamic biomarker of efficacy for PDPK1 inhibitors
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human PDPK1 phosphorylated at serine 241
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested WB-KO/KD - Validated
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: -A19011A was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100. While staining was observed, the signal was PDPK1-independent. We therefore do not recommend the clone for this application. -A19011A is predicted to recognize the residue corresponding to Ser241 in multiple PDPK1 isoforms due to the presence of the immunizing sequence in each isoform. -A19011A is predicted to recognize rat PDPK1 phosphorylated at Ser244 due to complete sequence homology between the immunizing peptide and the rat PDPK1 ortholog. -A19011A may cross-react with PSK1-α when phosphorylated at Ser181 due to partial sequence homology with the immunizing peptide
RRID: AB_2888741 (BioLegend Cat. No. 939601) AB_2888741 (BioLegend Cat. No. 939602)
Structure: PDPK1 is a 556 amino acid protein with a predicted molecular weight of 63 kD. Four other PDPK1 isoforms have been described, with predicted molecular weights of 48, 51, 58, and 60 kD.
Distribution: Ubiquitously expressed/cytosolic
Function: Serine-threonine protein kinase/cell signaling
Biology Area: Cell Biology
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Le Good JA, et al. 1998. Science. 281:2042. 2. Casamayor A, et al. 1999. Biochem J. 342 Pt2):287. 3. Dong LQ, et al. 2000. Proc. Natl. Acad. Sci. 97:5089. 4. Hinton HJ, et al. 2004. Nat. Immunol. 5:539. 5. Nagasima K, et al. 2005. J. Biol. Chem. 286:6433
Gene ID: 5170
UniProt: View information about PDPK on UniProt.org
Clone: A19011A
Regulatory Status: RUO
Other Names: PDK1, PkB Kinase, 3-Phosphoinositide Dependent Protein Kinase 1
Isotype: Mouse IgG1, κ