Biolegend, 939701, Purified anti-Gasdermin-D (N-terminus) Antibody, 25μg

Gasdermin D (N-terminus) is a member of the Gasdermin family of pore-forming proteins involved in pyroptosis. Gasdermin-D is vital to inducing pyroptotic cell death in response to pathogen and damage-associated molecular patterns, thus performing a role crucial to innate immune defense. Gasdermin-D is localized to the cytosol in an inactive state but, following cleavage into N and C-terminal fragments by inflammatory caspases Caspase-1, 4, or 5, the N-terminal fragment translocates to the plasma membrane where it forms large oligomeric pores. Pore-formation results in a disruption in the cell’s osmotic potential and subsequent cell lysis. Secretion of pro-inflammatory cytokines IL-1β and IL-18 from bone marrow dendritic cells (BMDCs), bone marrow derived macrophages (BMDMs), and neutrophils is also dependent upon Gasdermin-D, both through release by cell lysis and by direct passage through pores formed by its N-terminal fragment in the plasma membrane. Gasdermin-D exhibits bactericidal properties through the binding of its N-terminus to the cardiolipin of bacterial cell membranes and subsequent membrane perforation.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human Gasdermin-D corresponding to the N-terminus of the protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting and immunoprecipitation the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using NTERA-2 (negative control) and PC-3 (positive control) cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Both methods resulted in weak staining with high background. W18029B is predicted to recognize the pro-pyroptotic N-terminal fragment of cleaved Gasdermin-D. The immunogen used to generate this clone is fully contained within the sequence of the N-terminal fragment.
RRID: AB_2888893 (BioLegend Cat. No. 939701) AB_2888893 (BioLegend Cat. No. 939702)
Structure: Gasdermin-D is a 484 amino acid protein with an expected molecular weight of 53 kD.
Distribution: Ubiquitously expressed/Cytosol and plasma membrane
Function: Pyroptosis, inflammatory response
Biology Area: Innate Immunity, Mitochondrial Function
Molecular Family: Innate Immune Signaling
Antigen References: 1. Heilig R, et al. 2017. Eur. J. Immunol. 48:584. 2. Kambara H, et al. 2018. Cell Reports. 22:2924. 3. Mulvihill E, et al. 2018. EMBO J. 37:98321 4. Shi J, et al. 2015. Nature. 526:660.
Gene ID: 79792
UniProt: View information about Gasdermin-D on UniProt.org
Clone: W18029B
Regulatory Status: RUO
Other Names: GSDMD, DF5L, DFNA5L, GSDMC1,FKSG10, Gadermin domain-containing protein 1
Isotype: Rat IgG2b, λ