Biolegend, 941602, Purified anti-HuR (ELAVL1) Antibody, 100μg

Adenylate-uridylate-rich elements, or AREs, are short regions of RNA found in the 3’ UTR of messenger RNA. AREs are a major determinant of mRNA stability and translational efficiency, and play a crucial role in the post-transcriptional regulation of genes involved in several cellular processes. In humans, the Hu family of proteins is comprised of 4 members, each of which stabilize ARE-containing mRNA through directly binding to the ARE motif. In contrast to the Hu family members HuB, HuC, and HuD, which show neural specific distribution, HuR (also known as ELAVL1) is expressed in all tissues. While HuR is largely a resident nuclear protein under normal conditions, in response to cellular stress it can chaperone mRNA from the nucleus to the cytosol and continue to stabilize mRNA once in the cytosol. HuR is overexpressed and cytosolically delocalized in a wide range of cancers. In in vitro studies, HuR has been shown to stabilize several ARE-harboring mRNA encoding proteins involved in tumorigenesis and transformation, including MMP9, Bax, Fos, p21, and multiple cyclins, and is promising therapeutic target for cancer therapy.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human HuR protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100. While staining was observed with both methods, methanol permeabilization resulted in stronger HuR staining.
RRID: AB_2888897 (BioLegend Cat. No. 941601) AB_2888897 (BioLegend Cat. No. 941602)
Structure: HuR is a 326 amino acid protein with a predicted molecular weight of 36 kD.
Distribution: Ubiquitously expressed/Nucleus, cytosol, and stress granules
Function: mRNA stability, mRNA nuclear export/post-transcriptional gene regulation, embryonic development
Biology Area: Cell Biology
Antigen References: Peng SS, et al. 1998. EMBO J. 17:3461-70. Fan XC & Steitz JA. 1998. Proc Natl Acad Sci U S A. 95:15293-8. Wang W, et al. 2002. Mol Cell Biol. 22:3425-36.
Gene ID: 1994
UniProt: View information about HuR on UniProt.org
Clone: W18273D
Regulatory Status: RUO
Other Names: ELAV Like RNA Binding Protein 1, ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R), Embryonic Lethal, Abnormal Vision, Drosophilia, Homolog-Like 1, ELAV-Like Protein 1, Hu Antigen R, MeIG, Hua,
Isotype: Rat IgG2a, κ