Biolegend, 943403, PE anti-EGFR Phospho (Tyr845) Recombinant Antibody, 25tests

Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the protein. Phosphorylation at tyrosine 845, which is found in the kinase activation loop, stabilizes the active conformation and leads to the recruitment of adaptor proteins and other downstream effectors. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.
25tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human EGFR phosphorylated at tyrosine 845
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This clone was tested for ICC on A-431 cells treated with or without EGF. We failed to detect any EGFR Phospho (Tyr845) staining using the following fixation and permeabilization methods: 4% PFA plus Triton X-100 4% PFA plus methanol Methanol only This clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete sequence conservation with the immunizing peptide.
RRID: AB_2936764 (BioLegend Cat. No. 943403) AB_2936764 (BioLegend Cat. No. 943404)
Structure: EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
Distribution: Plasma membrane/Ubiquitously expressed
Function: EGFR signaling, cell signaling
Ligand/Receptor: EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF
Cell Type: Epithelial cells
Biology Area: Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Hunter T and Cooper JA. 1981. Cell. 24:741. 2. Gill GN and Lazar CS. 1981. Nature. 293:305. 3. Reynolds FH, et al. 1981. Nature. 292:259 4. Biscardi, et al. 1999. J. Biol. Chem. 274:8335. 5. Zhou M, et al. 2013. Cancer Res. 23:7056-67
Gene ID: 1956
UniProt: View information about EGFR on UniProt.org
Clone: QA19A93
Regulatory Status: RUO
Other Names: Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.