Biolegend, 944001, Purified anti-Hexokinase II Antibody, 25μg

Hexokinase enzymes are evolutionarily conserved proteins that play a critical role in glycolysis by catalyzing the conversion of glucose to the intermediate glucose-6-phosphate. In humans, four hexokinase isozymes exist that differ in their tissue distribution and affinity for glucose. The hexokinase II isozyme is ubiquitously expressed and is the most highly regulated member of the hexokinase family. In addition to its cytosolic localization, hexokinase II also associates with the mitochondrial outer membrane, resulting in efficient substrate channeling of ATP to the enzyme which fuels cell proliferation. Hexokinase II association with the outer mitochondrial membrane protein VDAC disrupts the ability of the pro-apoptotic protein Bax to induce mitochondrial dysfunction and cell death through cytochrome C release. Many cancer cells show elevated expression of hexokinase II, which allows efficient funneling of glucose to support anaerobic glycolysis, as well as increased survival through inhibition of Bax-mediated apoptotic death.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant hexokinase II protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: -This clone was tested for ICC on HeLa cells fixed and permeabilized with 4% PFA plus Trixon X-100, 4% PFA plus methanol, or methanol. The former two methods resulted in nuclear staining, inconsistent with the published cytosolic and mitochondrial outer membrane-associated localization of hekokinase II. The latter method (methanol only) resulted in hexokinase II staining, but the signal observed did not meet our sensitivity criteria. We therefore do not recommend this clone for the application. -This clone failed to immunoprecipitate hexokinase II from HeLa extracts. We therefore do not recommend this clone for the application.
RRID: AB_2890868 (BioLegend Cat. No. 944001) AB_2890868 (BioLegend Cat. No. 944002)
Structure: Hexokinase II is a 917 amino acid protein with a predicted molecular weight of 102 kD.
Distribution: Ubiquitously expressed; highly expressed in muscle and adipose tissue/Cytosolic, outer mitochondrial membrane-associated
Function: Glycolysis
Biology Area: Cell Biology, Mitochondrial Function
Molecular Family: Enzymes and Regulators
Antigen References: 1. Pastorino JG, et al. 2002. J. Biol. Chem. 277:7610 2. Majewski N, et al. 2004. Mol. Cell. 16:819
Gene ID: 3099
UniProt: View information about Hexokinase II on UniProt.org
Clone: W19163B
Regulatory Status: RUO
Other Names: HKII, Muscle Form Hexokinase
Isotype: Rat IgG2b, κ