Biolegend, 945102, Purified anti-SARS-CoV-2 S Protein S1 Antibody, 100μg

SARS-CoV-2 is a respiratory virus which causes coronavirus disease 2019 (COVID-19). The coronavirus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes. The S glycoprotein is synthesized as a precursor protein consisting of ~1,300 amino acids that is then cleaved into an amino (N)-terminal S1 subunit (~700 amino acids) and a carboxyl (C)-terminal S2 subunit (~600 amino acids). Three S1/S2 heterodimers assemble to form a trimer spike protruding from the viral envelope. The S1 subunit contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. Triggered by receptor binding, proteolytic processing and/or acidic pH in the cellular compartments, the class I viral fusion protein undergoes a transition from a metastable pre-fusion state to a stable post-fusion state during infection, in which the receptor-binding subunit is cleaved, and the fusion subunit undergoes large-scale conformational rearrangements to expose the hydrophobic fusion peptide, induce the formation of a six-helix bundle, and bring the viral and cellular membranes close for fusion. The trimeric SARS coronavirus (SARS-CoV-2) S glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to post-fusion conformation transition.
100μg
Verified Reactivity: SARS-CoV-2
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant SARS-CoV-2 S protein corresponding to S1 subunit
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested Direct ELISA, FC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For Direct ELISA, a concentration of 114.9 ng/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: BioLegend offers multiple clones that recognize SARS-CoV-2 S protein S1. Clone A20103H displayed the strongest performance for western blot of all clones validated for the application.
Product Citations: Mamedov T, et al. 2021. Viruses. 13:. PubMed
RRID: AB_2890875 (BioLegend Cat. No. 945101) AB_2890875 (BioLegend Cat. No. 945102)
Structure: Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 amino acids with a theoretical molecular weight of 141 kD, and consists of S1 and S2 subunits.
Distribution: Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
Function: Mediates fusion between virus and host cell membranes
Interaction: ACE2
Ligand/Receptor: ACE2
Biology Area: COVID-19
Antigen References: 1. Walls AC, et al. 2020. Cell. 181(2):281-292. 2. Yan R, et al. 2020. Science. 367 (6485):1444-1448. 3. Wrapp D, et al. 2020. Science. 367 (6483):1260-1263. 4. Shang J, et al. 2020. PNAS. 117(21):11727-11734
Gene ID: 43740568
UniProt: View information about Viral Protein on UniProt.org
Clone: A20103O
Regulatory Status: RUO
Other Names: S1, Spike Protein
Isotype: Rat IgG2a, κ