Biolegend, 947502, Purified anti-ERCC1 Antibody, 100μg

ERCC1, also referred to as Excision Repair Cross-Complementation Group 1, is a component of the nucleotide excision repair pathway. ERCC1 forms a heterodimer with XPF endonuclease to catalyze the 5’ incision in the process of excising a DNA lesion. ERCC1 also works with SLX4 in homology direct repair of double strand breaks and in the repair of crosslinks. Mutations in ERCC1 play a role in carcinogenesis due to genome instability, and elevated expression of ERCC1 is predictive of resistance to platinum-based chemotherapies in multiple types of cancers.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human ERCC1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.625 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W20045D detects multiple ERCC1 isoforms by western blot. Clone W20045D was tested for ICC using HeLa cells fixed with methanol or 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. All three methods were compatible with ERCC1 staining.
RRID: AB_2894534 (BioLegend Cat. No. 947501) AB_2894534 (BioLegend Cat. No. 947502)
Structure: ERCC1 is a 297 amino acid protein with an expected molecular weight of 33 kD. Four isoforms between 25 and 36 kD have been described.
Distribution: Ubiquitously expressed/ Nucleus
Function: DNA damage repair
Biology Area: Cell Biology, DNA Repair/Replication
Molecular Family: Enzymes and Regulators
Antigen References: Friboulet, et al. 2013. Cell Cycle. 12:3298 Kashiyama, et al. 2013. J Hum Genet. 92:807 Wan J, et al. 2017. Canc Invest. 35:85 Faridounnia, et al. 2018. Molecules. 23:3205
Gene ID: 2067
UniProt: View information about ERCC1 on UniProt.org
Clone: W20045D
Regulatory Status: RUO
Other Names: Excision Repair Cross-Complementation Group 1, ERCC excision repair 1, endonuclease non-catalytic subunit, RAD10
Isotype: Rat IgG2b, κ