Biolegend, 947903, Ultra-LEAF™ Purified anti-mouse IL-13Rα2 (BA) Antibody, 100μg

IL-13 is a key mediator in the pathogenesis of allergic inflammation. Its receptor is comprised of IL-4Rα and IL-13Rα1, which transduct the signaling to the downstream STAT6 pathway. IL-13Rα2, also known as IL-13Ra2 and IL-13BP, serves as a decoy receptor to attenuate IL-13-induced response. IL-13Rα2 binds IL-13 with 100-300 folds higher affinity than does IL-13Rα1, but IL-13Rα2 lacks signaling motifs on its cytoplasmic tail. Mice deficient in IL-13Rα2 showed increased IgE level and IFNγ production, consistent with a phenotype of enhanced IL-13 effects. Airway hyperresponsiveness and inflammation was enhanced in IL-13Rα2 transgene mice, indicating that IL-13Rα2 blocks IL-13 mediated response. In mice, there are two discrete transcripts generated by alternative splicing, producing membrane-bound and secreted forms, respectively. Some evidences indicated that MMP-8 contributes to soluble forms of IL-13Rα2 by cleaving membrane-bound IL-13Rα2. In human, only membrane-bound transcript was found and soluble forms were absent in human plasma.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse IL-13Rα2-Fc chimera
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the inhibition of proliferation induced by recombinant mouse IL-13Rα2-Fc chimera (Cat. No.770704) at 0.05 µg/mL on human TF-1 cells. ND50 range: 0.01-0.06 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2922648 (BioLegend Cat. No. 947903) AB_2922648 (BioLegend Cat. No. 947904)
Structure: Disulfide bond-linked homodimer
Distribution: Spleen, brain; membrane-bound and soluble form
Function: IL-13Rα2 serves as a decoy receptor to inhibit IL-13-induced responses.
Ligand/Receptor: IL-13
Cell Type: B cells, Fibroblasts
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines, Innate Immune Signaling, Soluble Receptors
Antigen References: Zhang JG, et al. 1997. J Biol Chem. 272: 9474-80. Donaldson DD, et al. 1998. J Immuno. 161: 2317-24. Kawakami K, et al. 2001. Blood. 97: 2673-9. Wood N, et al. 2003. J Exp Med. 197: 703-9. Chen W, et al. 2014. J Allergy Clin Immunol. 132: 951-8. Chen W, et al. 2008. J Allergy Clin Immunol. 122: 625-32. Chen W, et al. 2009. J Immunol. 183: 7870-6.
Gene ID: 16165
UniProt: View information about IL-13Ralpha2 on UniProt.org
Clone: W19369C
Regulatory Status: RUO
Other Names: Interleukin-13 receptor subunit alpha-2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, IL-13R alpha-2, IL-13Rα2, IL-13Ra2, CD213a2, IL-13 binding protein, IL-13BP
Isotype: Rat IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.