Biolegend, 948002, Purified anti-Cathepsin B Antibody, 100μg

Cathepsin B (CTSB) is a lysosomal cysteine protease. While most cathepsins are exclusively endopeptidases, CTSB exhibits both carboxypeptidase and endopeptidase activities. The optimal pH for CTSB activity is between four and six. Cystatin C has been identified as an endogenous CTSB inhibitor. High CTSB protein levels and activity have been found in many tumors including breast, cervix, colon, stomach, glioma, lung, and thyroid. CTSB can be secreted by tumor cells and is associated with the cell membrane of these cells. Membrane associated CTSB promotes extracellular matrix (ECM) degradation, which contributes to cancer motility and invasion. Many ECM proteins, including laminin, fibronectin, and collagen IV, are substrates of CTSB. CTSB can also activate pro-uPA/PLAU. Activated uPA promotes ECM digestion through serine protease plasminogen. It has been shown that the inhibition of CTSB can limit bone metastasis in breast cancer, which makes it an important anti-cancer drug target. CTSB has been proposed as a new drug target for Alzheimer's disease because of its involvement in the production of neurotoxic β-amyloid (Aβ) peptides. The inhibition of CTSB can reduce the brain Aβ peptides and improve memory that was determined from a mouse model of Alzheimer's disease. CTSB also plays significant roles in immune responses including both T and B cell apoptosis and Th1/Th2 polarization. CTSB is implicated in other pathological conditions including cardiovascular disease, multiple sclerosis, and arthritis. CTSB may also have a role in autophagy, adipogenesis, and cholesterol absorption in the intestine.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human cathepsin B protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IHC-P- Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W20149K detects both pro-Cathepsin B and heavy chain cathepsin B by western blot.Clone W20149K was tested for ICC using the following fixation-permeabilization methods: 4% PFA + Triton X-100, 4% PFA + methanol, and methanol-only. Of these, methanol only was compatible with cathepsin B staining, while 4% PFA + Triton X-100 or methanol resulted in weak/absent staining of cathepsin B.
RRID: AB_2904460 (BioLegend Cat. No. 948001) AB_2904460 (BioLegend Cat. No. 948002)
Structure: Cathepsin B is a 339 amino acid protein with a predicted molecular weight of 37.8 kD.
Distribution: Ubiquitously expressed/Lysosome
Function: Lysosomal cysteine protease involved in intracellular protein turnover
Interaction: Interacts with Hepatitis B spliced protein, Bikunin, and TSRC1.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience
Molecular Family: Lysosomal Markers, Proteases
Antigen References: Mohamed MM, Sloane BF. 2006. Nat. Rev. Cancer 6:764. Wang C, et al. 2012. J. Biol. Chem. 47:39834. Grzonka Z, et al. 2001. Acta Biochim. Pol. 48:1. Liu J, et al. 2006. FEBS Letters 580:245. Brix K, et al. 2008. Biochimie. 90:194. Turk V, et al. 2012. Biochim. Biophys. Acta. 1824:68.
Gene ID: 1508
UniProt: View information about Cathepsin B on UniProt.org
Clone: W20149K
Regulatory Status: RUO
Other Names: CTSB, CPSB, APP secretase, APPS, Cathepsin B1
Isotype: Rat IgG2b, κ