Biolegend, 948101, Purified anti-Alpha β Crystallin Antibody, 25μg

Alpha B Crystallin (CryAB) is a member of the small heat shock protein (HSP20) family.  Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. CryAB can be induced by heat shock, ischemia and oxidation. The protein is known to protect cells from stress damage. It may also play an important role in the Golgi reorganization during cell division. CryAB is also known to be associated with a number of neurodegenerative diseases such as Alzheimer’s disease and has been implicated in breast tumors of patients with shorter survival, suggesting that it may be a useful marker in identifying aggressive breast cancer.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant human alpha β crystallin protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone recognizes alpha β crystallin proteins at 21 kD in human, mouse, and rat brain lysates. An extra band at 15 kD is observed in the human brain lysates.
RRID: AB_2904461 (BioLegend Cat. No. 948101) AB_2904461 (BioLegend Cat. No. 948102)
Structure: Alpha β crystallin is a 175 amino acid protein with a predicted molecular weight of 21 kD.
Distribution: Ubiquitously expressed /Cytosol
Function: Has chaperone-like activity
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Antigen References: Erin-Mai FL, et al. 2021. J Neuroinflammation. 18:82. Zhihui Z and Georg R. 2018. Neurochem Int. 115:69-79. Raman B, et al. 2015. BioChim Biophys Acta. 1854:291-319. Julie A, et al. 2012. Int J Biochem Cell Biol. 44:1622-31.
Gene ID: 1410
UniProt: View information about Alpha beta Crystallin on UniProt.org
Clone: 1329CT523.140.120
Regulatory Status: RUO
Other Names: Crystallin Alpha B, alpha-crystallin B chain, CRYAB, Heat Shock Protein Beta-5, HspB5, Renal Carcinoma Antigen NY-REN-27
Isotype: Mouse IgG1, κ