Biolegend, 948401, Purified anti-P-Cadherin Antibody, 25μg

P-cadherin, or placental-cadherin, belongs to one of the three subclasses in the cadherin family. Cadherins are Ca2+ dependent adherent proteins involved in intercellular cell adhesions. P-cadherin contains a cytoplasmic carboxy-terminal domain, a transmembrane domain, and a extracellular N-terminal domain. P-cadherin was first identified in mouse placental tissue. However, in humans, the distribution of P-cadherin is restricted to the basal or lower layers of the stratified epithelia in the epithelial layer, and is not found in human placental tissue. In humans, the loss of functional P-cadherin can induce characteristic genetic syndromes such as hypotrichosis with juvenile muscular dystrophy (HJMD) and ectodermal dysplasia, ectrodactyly, and muscular dystrophy (EEM syndrome). In breast cancer, an overexpression of P-cadherin is often found in high histological grade tumors. In an in vitro study, the overexpression of P-cadherin in breast cancer cells in the presence of wild type E-cadherin , promoted cell invasiveness, motility, and migration.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full-length Human P-cadherin peptide.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using 4% PFA-fixed A-431 cells permeabilized with either methanol or Triton X-100. Neither method was compatible with P-Cadherin staining. This clone was also tested using methanol fixation on A-431 and HaCaT cells. Neither cell line was compatible with P-Cadherin staining.
RRID: AB_2894538 (BioLegend Cat. No. 948401) AB_2894538 (BioLegend Cat. No. 948402)
Structure: P-Cadherin is a 829 amino acid protein with a predicted molecular weight of 91 kD.
Distribution: Adhesion protein/Plasma membrane
Function: Intercellular cell adhesion
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure
Antigen References: Ribeiro A. et al. 2010. Oncogene. 29:392-402. Shimoyama Y. and Hirohashi S. 1991. Cancer Research. 51:2185-2192. Sun L. et al. 2011. American Journal of Pathology. 179:380-390. Vieira A. & Paredes J. 2015. Molecular Cancer. 14:178.
Gene ID: 1001
UniProt: View information about P-Cadherin on UniProt.org
Clone: 12H6
Regulatory Status: RUO
Other Names: Cadherin 3, CDH3, Placental Cadherin
Isotype: Mouse IgG1, κ