Biolegend, 948703, Alexa Fluor® 647 anti-CHOP Antibody, 25tests

When unfolded proteins accumulate in the ER lumen, multiple unfolded protein response (UPR) pathways become activated, including PERK, ATF6, and IRE1α, all of which transcriptionally reprogram the flux of proteins through the ER. When these quality control mechanisms fail to restore ER homeostasis, sustained stress results in the induction of CHOP, a pro-apoptotic transcription factor that negatively regulates expression of BCL2 and increases expression of BIM and TNFS10RB. One mechanism cancer cells exploit to adapt to harsh conditions of the tumor microenvironment is through upregulation of UPR pathways. Thus, understanding the molecular mechanisms that govern the switch from adaptation to apoptosis is of great therapeutic interest.
25tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant human CHOP protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This clone failed to detect CHOP in human small intestine. For ICC testing, we do not recommend methanol only as a fixation-permeabilization step due to poor CHOP staining. We recommend 4% PFA fixation with either Triton X-100 or methanol permeabilization.
RRID: AB_2936753 (BioLegend Cat. No. 948703) AB_2936753 (BioLegend Cat. No. 948704)
Structure: CHOP is a 169 amino acid protein with a predicted molecular weight of 19 kD.
Distribution: Expressed in cells and tissue undergoing ER stress/Nucleus
Function: Transcription Factor
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Misfolding and Aggregation, Transcription Factors
Antigen References: 1. Yamaguchi, et al. 2004. J. Biol. Chem. 279: 45495 2. Oyadomari, et al. 2004. Cell Death Differ. 11:381. 3. Puthalakath H, et al. 2007. Cell. 129: 1337.
Gene ID: 1649
UniProt: View information about CHOP on UniProt.org
Clone: 9C8/CHOP
Regulatory Status: RUO
Other Names: DDIT3, GADD153
Isotype: Mouse IgG2b, κ