Biolegend, 987702, APC/Fire™ 750 anti-human CD49d, 500microl

CD49d is a 150 kD α integrin chain known as α4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α4β7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation.
500microl
Reactivity: Human
Formulation: Phosphate-buffered solution, pH7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 200 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: Suggested for Flow Cytometry
Disclaimer: WARNINGS AND PRECAUTIONS Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations. Do not use this reagent beyond the expiration date stated on the label. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration. Avoid prolonged exposure of the reagent or stained cells to light.
Antigen References: Elices M, Ed. 1995. Springer Semin. Immunopathol. 16(4). Lobb RR and Helmer ME. 1994. J. Clin. Invest. 94:1722-8.
Clone: 9F10
Workshop: V S215
Other Names: VLA-4 α chain, α4 integrin, Integrin α4 chain, ITGA4
Isotype: Mouse IgG1, κ