CST, 2325S, Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for WB,IP,IHC,E. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit Monoclonal Antibody (CST #2325) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 Peptide ELISA (DELFIA): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit Monoclonal Antibody detects phospho-serine in a PXS*P or S*PXR/K motif, as well as a PXS*PXR/K motif. This antibody is phospho-specific, and does not react with phospho-threonine- or phospho-tyrosine-containing peptides/proteins. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with synthetic phospho-MAPK/CDK substrate peptides . Background The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P, and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXR/K. Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline, motifs PXS*/T*P and/or S*PXR/K, for use in the study and discovery of new MAPK and CDK substrates (4,5). Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG