CST, 31452S, Transportin-1 (E7X4Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IPO2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Transportin-1 (E7X4Z) Rabbit Monoclonal Antibody (CST #31452) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Transportin-1 (E7X4Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total transportin-1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala354 of human transportin-1 protein. Background Transportin-1 (TNPO1), also known as MIP or karyopherin-β2, is a nuclear import protein that binds to nuclear localization signals (NLSs) on various proteins to help shuttle them from the cytoplasm to the nucleus (1-3). While initially discovered to shuttle hnRNP proteins, transportin-1 has also been shown to import other important regulatory proteins, such as c-Fos and ADAR1 (1,4,5). Transportin-1 plays a role in HIV replication, as it imports and helps uncap the capsid protein (6). Transportin-1 has been implicated in familial ALS, due to its impaired binding affinity to mutations in the FUS-NLS region. Reduction in transportin-1 binding correlated with the time of ALS disease progression in these mutants (7). Transportin-1 can also accumulate in FUS inclusions in wild-type FUS ALS (8). Additionally, transportin-1 plays a role in circadian rhythms by importing PER1 to the nucleus (9). Alternate Names importin 2; importin beta 2; Importin beta-2; IPO2; karyopherin (importin) beta 2; Karyopherin beta-2; KPNB2; M9 region interaction protein; MIP; MIP1; TNPO1; transportin 1; Transportin-1; TRN Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG