CST, 3406S, Phospho-CSF-1R/M-CSF-R (Tyr923) Antibody

Polyclonal Antibody for studying CSF1R (Tyr923) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-CSF-1R/M-CSF-R (Tyr923) Antibody (CST #3406) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-CSF-1R/M-CSF-R (Tyr923) Antibody detects endogenous levels of CSF-1R/M-CSF-R only when phosphorylated at Tyr923. This antibody may cross-react with other activated tyrosine kinases including PDGF and FGF receptors. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr923 of human CSF-1R/M-CSF-R. Antibodies are purified by protein A and peptide affinity chromatography. Background Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation, and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8). The equivalent site (Tyr921) of Tyr923 in human M-CSF receptor was demonstrated to be phosphorylated in mouse macrophages in a CSF-1 stimulation dependent manner (10). Phosphorylation of Tyr923 of M-CSF receptor may provide a docking site for Grb2 binding (9). Alternate Names BANDDOS; C-FMS; CD115; CD115 antigen; colony stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; CSF-1R; CSF1R; CSFR; FIM2; FMS; FMS proto-oncogene; HDLS; M-CSF-R; macrophage colony stimulating factor I receptor; Macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; Proto-oncogene c-Fms Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 175 SOURCE: Rabbit